Program
Sunday May 22
14:00-15:15 Registration open
15:15 Opening
Chair: Marcel Tijsterman
15:20-16:00 Tom Kunkel (NIH, USA)
”Eukaryotic DNA Replication Fidelity by the B-Family DNA Polymerases”
16:00-16:30 Joanne Sweasy (University of Arizona, USA)
”Fidelity Mechanisms of DNA Polymerase Beta”
16:30-17:00 Wei Yang (The National Institutes of Health, USA)
“Flexible substrate binding and multiple deprotonation paths of DNA polymerases”
17:00-17:30 Coffee/tea break
17:30-18:00 Katarzyna Bebenek (NIEHS, USA)
”Structural Insights into How Polymerase Lambda Engages DNA Double Strand Breaks”
18:00-18:20 Erik Johansson (Umeå University, SE)
”In crystallo study of the reaction mechanism in a family B DNA polymerase”
18:40 Dinner at the Wenner-Gren Centre
Monday May 23
Chair: Sjoerd Wanrooij
09:00-09:30 William Copeland (NIEHS, USA)
“New insights into mitochondrial DNA replication”
09:30-09:50 Josefin Forslund (Umeå University, SE)
”The disease mechanism of DNA polymerase gamma Y951N mutation”
09:50-10:10 María Isabel Martínez-Jiménez (Centro de Biología Molecular Severo Ochoa, ES)
“Human PrimPol evades ddCTP and tenofovir nucleotide analogs and relieves mitochondrial toxicity”
10:10-10:50 Coffee/tea break
10:50-11:20 Maria Falkenberg (University of Gothenburg, SE)
“Molecular basis of mitochondrial DNA replication in mammalian cells”
11:20-11:40 Margaret Gustafson (NIEHS, USA)
“Mechanisms of SSBP1 variants in mitochondrial disease: Molecular dynamics simulations reveal stable tetramers with altered DNA binding surfaces”
11:40-12:00 Anders Clausen (University of Gothenburg, SE)
“Stably incorporated ribonucleotides in murine tissues: quantitation, base identity and distribution in nuclear and mitochondrial DNA”
12:00-13:50 Lunch
Chair: Julian Sale
13:50-14:20 Polina Shcherbakova (University of Nebraska, USA)
”DNA polymerase ε variants in cancers: new insights into the mechanisms of pathogenicity”
14:20-14:50 Zac Pursell (Tulane University, USA)
“Insights Into Polymerase-Mediated Mutagenesis and Tumorigenesis From Mouse Models, Cell Culture and Human Patients”
14:50-15:10 Sean Rudd (Karolinska Institute, SE)
”Inhibiting the dNTP hydrolase SAMHD1”
15:10-15:30 Andrei Chabes (Umeå University, SE)
“Regulation of dNTP pools in response to DNA damage”
15:30 Coffee/tea
18:15 Transport to Vasa Museum
19:00 Guided tour and Dinner (around 20:00) at the Vasa Museum
Tuesday May 24
Chair: Peter Burgers
09:00-09:30 Meindert Lamers (Leiden University, Medical Center, NL)
“A four-point molecular hand-off during Okazaki fragment maturation in E. coli“
09:30-09:50 Ceslovas Venclovas (Vilnius University, LT)
”New insights obtained from computational analysis of DNA polymerases in complete bacterial genomes”
09:50-10:10 Janice Pata (University of Albany, USA)
”Kinetic mechanisms of Staphylococcus aureus DNA polymerases PolC and DnaE: Insights into the initiation of DNA synthesis in Gram-positive bacteria”
10:10-10:30 Ignacio Mir Sanchis (Umeå University, SE)
“DNA polymerases in the Staphylococcal metaverse”
10:30-10:50 Coffee/tea break
Chair: Erik Johansson
10:50-11:20 Wolf-Dietrich Heyer (University of California, USA)
”BRCA2 and RAD52 inhibit DNA Polymerase q to enforce DSB repair pathway choice”
11:20-11:40 Kei-ichi Takata (Institute for Basic Science, KR)
“DNA polymerase theta mediated repair of high LET radiation-induced DNA double strand breaks”
11:40-12:00 Joost Schimmel (Leiden University, Medical Center, NL)
“CST-guided Polymerase a-primase action at DNA breaks induces small tandem duplications and prevents large deletions”
12:00-13:50 Lunch
13:50-14:20 Sylvie Doublié (University of Vermont, USA)
“Structural and biochemical investigation of Pol θ’s unique functions”
14:20-14:50 Marcel Tijsterman (Leiden University, NL)
“Polymerase theta-mediated end joining biology”
14:50-15:10 Andrea Kaminski (NIEHS, USA)
“Structural snapshots of human DNA polymerase µ engaged on a DNA double-strand break”
15:10-18:30 Poster viewing
18:30 Dinner at the Wenner-Gren Center
Wednesday May 25
Chair: Wolf-Dietrich Heyer
09:00-09:40 John Diffley (Francis Crick Institue, UK)
“Understanding Eukaryotic Chromosome Replication through Biochemical Reconstitution”
09:40-10:00 Giovanni Maga (Institute of Molecular Genetics IGM CNR, IT)
“The Jigsaw puzzle of hybrid RNA-DNA genome tolerance pathways”
10:00-10:20 Kelly Zatopek (New England Biolabs)
“Unmasking the Ribonucleotide Steric Gate in Family D DNA Polymerases”
10:20-10:50 Coffee/tea break
Chair: Polina Shcherbakova
10:50-11:20 Peter Burgers (Washington University St Louis, USA)
“The lame leading the blind: Rev1-DNA polymerase zeta in TLS”
11:20-11:50 Vincent Pages (CNRS, FR)
“Control of TLS DNA polymerases in response to genotoxic stress: global or local regulation?”
11:50-12:10 Dmitry Korzhnev (University of Connecticut Health Center, USA)
“Evolution of Rev7 interactions in eukaryotic TLS DNA polymerase Pol”
12:10-14:00 Lunch
Chair: Andrei Chabes
14:00-14:30 Luis Blanco (Centro de Biología Molecular Severo Ochoa, ES)
“The mechanism of DNA primer synthesis by human PrimPol”
14:30-14:50 Sjoerd Wanrooij (Umeå University, SE)
“PolDIP2 enhances nucleotide binding and DNA synthesis by PrimPol”
14:50-15:10 Tatiana Moiseeva (Tallinn University of Technology, EE)
“Using mAID-mediated depletion of DNA polymerase epsilon to uncover its non-catalytic role in human cell”
15:10-15:30 Sellés-Baiget Selene (NNF Center for Protein Research, DK)
“Non-catalytic function of DNA polymerase κ during lesion bypass”
15:30-16:00 Coffee/tea break
16:00-16:30 Julian Sale (MRC Cambridge, UK)
“The mutagenic footprint of DNA replication origins”
16:30-17:00 Pat Foster (Indiana University Bloomington, USA)
”Insights into the fidelity of DNA replication gained from mutation accumulation studies in Escherichia coli”
17:00-17:20 Ludovic Sauguet (Institut Pasteur, FR)
“New Insights on the D-family archaeal replicative DNA polymerase: structure, evolution and interactions with its replisome partners”
17:20-17:40 Tahir Tahirov (University of Nebraska Medical Center, USA)
“Mechanism of Primase-Polalpha termination”
17:40-17:50 Erik Johansson and Sjoerd Wanrooij “on the 7th DNA Polymerases Meeting” and closure
18:00 Dinner at Wenner Gren Center
Thursday May 26
Departure
6th DNA Polymerases Meeting 